OVERVIEW

The 2014 Workshop on Quantitative Microscopy is an intensive study of the techniques and principles used in stereology and image analysis.  This is a unique opportunity to interact with experts in the field of stereology and image analysis.  In addition students will be introduced to processes that that will dramatically improve efficiency and accuracy of stereology and image analysis.  The workshop is five days of lectures, interactive sessions and hands-on experience.

WORKSHOP GOALS

  1. Participants will learn how to design and conduct studies for stereology and image analysis.
  2. Participants will learn the theories behind the use of stereology to accurately measure specific histological and/or histopathological features in an organ and tissue sections.
  3. Participants will be exposed to digital imaging on several state-of-the-art imaging platforms including the Hamamatsu  NanoZoomer® Digital Pathology Slide Scanner.
  4. Participants will work with the faculty to develop protocols for analysis of their own digital images that will be generated during the workshop.

MAJOR TOPICS INCLUDE

STEREOLOGY

  • Optical dissector/fractionator
  • Physical dissector/fractionator
  • Cavalieri’s estimator for volume
  • Cycloids/Lines for surface
  • Counting frames/Global Spatial Sampling for length
  • Connectivity, Tensors and Spatial distribution
  • Nucleator and Rotators for Local Stereology
  • Virtual Slide-based Stereology
  • New Estimators & Methods
  • Automated Physical Disector/fractionator
  • Proportionator
  • Tissue deformation
  • Ratios vs Totals
  • How many Animals/Blocks/FOVs and counts?

IMAGE ANALYSIS

  • Image processing: filters, color de-convolution
  • Image segmentation: Thresholding, advanced pixel classifiers - Bayesian and K-means, contextual classifiers
  • Limitations of image analysis
  • Tissue Micro Array image analysis
  • Tissue Sampling
  • Image analysis protocols for sampling
  • Immunohistochemical biomarker co-localization
  • Virtual Slide based Image Analysis

WHO SHOULD ATTEND

This workshop is for individuals that are interested in making meaningful quantitative measurements from digital images captured with various types of microscopes.  This course is intended for students, post-docs, and researchers from the fields of biology, pathology, and medicine.  It will be useful for researchers in academic laboratories and in the biopharmaceutical industry.  The course will provide a detailed overview in the theory and practice of geometric sampling, measurement of 3D structures (stereology), and image analysis.  The course structure will include lectures on the theory of stereology and image analysis, problem-solution exercises, and hands on experience with image analysis software.  Participants are invited to bring digital images, glass slides, or PowerPoint presentations from their research to work with the course faculty on specific applications or problems.

THE FACULTY

Jens Randell Nyengaard, M.D., Dr. of Medicine, Professor at the Stereology and EM Research Laboratory at the University of Aarhus, Denmark.

Johnnie Bremholm Andersen, Ph.D., Honorary Associate Professor at the Stereology and EM Research Laboratory at the University of Aarhus, Denmark.

Lars Pedersen, Ph.D., Visiopharm A/S, Agern Allé 3, DK-2970 Hørsholm, Denmark

Charles W. Frevert, D.V.M., Sc.D.,  Research Associate Professor from the University of Washington, Director of the Histology and Imaging Core at South Lake Union.

Brian Johnson, Program Manager, Histology and Imaging Core, University of Washington at South Lake Union

LOCATION

The workshop will be held at:

University of Washington School of Medicine at South Lake Union
850 Republican Ave.
Seattle, WA 98109

The Workshop will begin Monday Morning, October 27th at 9:00 AM and will end at 3:00 PM on October 31st.

To reserve your hotel room, please call 800.228.9290 or 800.321.2211 and ask for the Workshop on Quantitative Microscopy room block at the Courtyard Seattle Lake Union Hotel.  Or use the online reservation link here:  Book Your Group Rate for Workshop on Quantitative Microsopy

Group Block Information:  Courtyard Seattle Downtown/Lake Union from October 26-31 for $139.00 plus tax per night for a king.  Current parking fee of $25 overnight per vehicle.  Cutoff date for reservations is October 3, 2014 to receive the group rate.

REGISTRATION

The registration fee is $1,000 for academic and $2,000 for industry and includes daily lunch, snacks, dinner on October 29th at 7pm and all instructional materials.

Participants are responsible for their own hotel reservations, arranging transportation to and from the hotel, airfare accommodations, and evening meals.

Please register online by clicking here for the Registration Portal.

Credit card payment is due at time of registration.  Registration will be confirmed by e-mail on a first-come, first-served basis.

PARTICIPANTS

HISTOLOGY AND IMAGING CORE; THE COMPARATIVE PATHOLOGY PROGRAM; DEPARTMENT OF COMPARATIVE MEDICINE
UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE AT SOUTH LAKE UNION    
STEREOLOGY AND EM RESEARCH LABORATORY
AARHUS UNIVERSITY
   
VISIOPHARM
THE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY  
THE HISTOCHEMICAL SOCIETY